Synthesis of low immunogenicity RNA with high-temperature in vitro transcription
نویسندگان
چکیده
منابع مشابه
Synthesis of RNA by in vitro transcription.
In vitro transcription is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using th...
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ژورنال
عنوان ژورنال: RNA
سال: 2020
ISSN: 1355-8382,1469-9001
DOI: 10.1261/rna.073858.119